Perbedaan Profil Mikrobiota Usus pada Kasus Hirschsprung, Hirschsprung-Associated Enterocolitis, dan Non-hirschsprung

Lulik Inggarwati; Oei Thomas Sanjaya  Wibowo; Dewi Santosaningsih; Harun Al Rasyid; Happy Kurnia Permatasari; Wisnu Barlianto; Respati Suryanto Dradjat

doi:10.11594/jk-risk.06.1.3

Authors

Lulik Inggarwati Pediatric Surgery Division, Department of Surgery, Faculty of Medicine Universitas Brawijaya, Dr. Saiful Anwar Hospital, Malang, East Java, Indonesia
Oei Thomas Sanjaya Wibowo General Surgery Resident, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia
Dewi Santosaningsih Department of Clinical Microbiology, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia
Harun Al Rasyid Department of Public Health, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia
Happy Kurnia Permatasari Department of Biochemistry and Biomolecular Biology, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia
Wisnu Barlianto Department of Pediatrics, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia
Respati Suryanto Dradjat Department of Orthopaedics and Traumatology, Faculty of Medicine Universitas Brawijaya, Malang, East Java, Indonesia

DOI:

https://doi.org/10.11594/jk-risk.06.1.3

Keywords:

Hirschsprung, enterocoliis, microbiota

Abstract

Background: It is estimated that approximately 939 babies are born each year with Hirschsprung disease. Hirschsprung-Associated Enterocolitis (HAEC) is a complication of HSCR and represents the leading cause of morbidity and mortality in these patients. An abnormal composition of intestinal microorganisms is one of the risk factors for the development of HAEC.

Aim: This study aims to determine the differences in the ratios of Clostridium spp. and Escherichia coli, as well as the intestinal microbiota phylum profiles, among HSCR, HAEC, and non-HSCR cases.

Methods: This cross-sectional study involved three groups, namely HSCR, HAEC, and non-HSCR, with six patients in each group. The ratio between Clostridium spp. and Escherichia coli was determined using stool culture. The intestinal microbiota profiles were analyzed through 16S rRNA sequencing. The composition ratios of gut microbiota were statistically analyzed using IBM SPSS version 22.0 (Armonk, NY, USA).

Results: A significant difference was found in the ratio of Escherichia coli/Clostridium spp. among the three groups (p= 0.017). Among the phyla, only Bacteroidetes showed a significant difference (p= 0.010), while Actinobacteria, Firmicutes, and Proteobacteria did not differ significantly, with p-values of 0.948, 0.081, and 0.207, respectively.

Conclusion: Significant differences in the Escherichia coli/Clostridium spp. ratio and the phylum Bacteroidetes among study groups reflect gut microbiota dysbiosis associated with HAEC.

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