Correlation Between Antimicrobial Resistance and Biofilm Formation Ability of Pseudomonas aeruginosa Isolates from Sputum ETT in the Intensive Care Unit of Dr. Saiful Anwar Hospital
DOI:
https://doi.org/10.11594/jk-risk.05.2.3Keywords:
Biofilm, Pseudomonas aeruginosa;, Antimicrobial resistance, Sputum ETT, ICUAbstract
Background: Pseudomonas aeruginosa is a prominent pathogen responsible for hospital-acquired infections. Its pathogenic potential is primarily driven by antimicrobial resistance and the ability to form biofilms.
Aim: To examine the correlation between antimicrobial resistance patterns and the biofilm-forming ability of Pseudomonas aeruginosa isolated from endotracheal tube (ETT) sputum specimens.
Methods: This analytic cross-sectional study used sputum samples from ICU patients with ETT. Biofilm detection was done using the microtiter plate method, and antimicrobial susceptibility was tested using Vitek.
Results: Of the 23 multidrug-resistant (MDR) isolates identified, 45.5% demonstrated biofilm-forming ability, while 54.5% did not. Statistical analysis using the Chi-square test revealed no significant association between antimicrobial resistance and biofilm formation.
Conclusion: There was no significant correlation between multidrug resistance and biofilm production in Pseudomonas aeruginosa isolates from ETT sputum samples.
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2. Rather M, Gupta K, Mandal M. Microbial biofilm: Formation, resistance, and control. Braz J Microbiol. 2021;52:1701–18.
3. Thorarinsdottir H, Kander T, et al. Biofilm formation on endotracheal tubes: A clinical trial. Crit Care. 2020;24:382. https://doi.org/10.1186/s13054-020-03092-1
4. Li Y, Roberts JA, Walker MM, et al. Global epidemiology of VAP caused by MDR Pseudomonas aeruginosa: A systematic review. Int J Infect Dis. 2023. https://doi.org/10.1016/j.ijid.2023.11.023
5. Abebe G. The role of bacterial biofilm in antibiotic resistance and food contamination. Int J Microbiol. 2020; Article ID 1705814. https://doi.org/10.1155/2020/1705814
6. Abbas H, Serry FM, Masry EM. Combating Pseudomonas aeruginosa biofilm by potential biofilm inhibitors. Asian Pharma Press. 2012;2(2):66–72.
7. Thi M, Wibowo D, Rehm B. Pseudomonas aeruginosa biofilms. Int J Mol Sci. 2020;21(22):8671. https://doi.org/10.3390/ijms21228671
8. Liao C, Huang X, Wang Q, et al. Virulence factors of Pseudomonas aeruginosa and antivirulence strategies. Front Cell Infect Microbiol. 2022;12:926758. https://doi.org/10.3389/fcimb.2022.926758
9. Haidar A, Muazzam A, Nadeem A, et al. Biofilm formation and antibiotic resistance in Pseudomonas aeruginosa. The Microbes. 2024. https://doi.org/10.1016/j.microb.2024.100078
10. Karami P, Khaledi A, Mashoof R, et al. Correlation between biofilm formation capability and antibiotic resistance in Pseudomonas aeruginosa. Gene Rep. 2019. https://doi.org/10.1016/j.genrep.2019.100561
11. Ochoa S, Cordova A, Rodea G, et al. Characterization of MDR P. aeruginosa from pediatric patients and biofilm formation. Microbiol Res. 2015. http://dx.doi.org/10.1016/j.micres.2014.11.005
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